Recellularized human dermis for testing gene electrotransfer ex vivo

Thermal Assisted In Vivo Gene Electrotransfer.

Gene electrotransfer is an effective approach for delivering plasmid DNA to a variety of tissues. Delivery of molecules with electric pulses requires control of the electrical parameters to achieve effective delivery. Since discomfort or tissue damage may occur with high applied voltage, the reduction of the applied voltage while achieving the desired expression may be an important improvement....

Effect of temperature on the optical properties of ex vivo human dermis and subdermis.

The effect of temperature on the optical properties of human dermis and subdermis as a function of near-infrared wavelength has been studied between 25 degrees C and 40 degrees C. Measurements were performed ex vivo on a total of nine skin samples taken from the abdomen of three individuals. The results show a reproducible effect of temperature on the transport scattering coefficient of dermis ...

construction and evaluation of a specific nonviral vector for ex-vivo gene correction of human β-globin gene defects

Effects of a skin-massaging device on the ex-vivo expression of human dermis proteins and in-vivo facial wrinkles

Mechanical and geometrical cues influence cell behaviour. At the tissue level, almost all organs exhibit immediate mechanical responsiveness, in particular by increasing their stiffness in direct proportion to an applied mechanical stress. It was recently shown in cultured-cell models, in particular with fibroblasts, that the frequency of the applied stress is a fundamental stimulating paramete...

Ex vivo adenovirus mediated gene transfection of human conjunctival epithelium.

AIM To investigate the efficacy of "ex vivo" adenoviral vector mediated gene transfection of human conjunctival epithelial cell as a possible route for gene therapy for the distribution of anti-inflammatory agents for the potential treatment of immune mediated ocular inflammatory disorders. METHODS Human conjunctival cells (HCs) were cultured with various concentrations of recombinant adenovi...